prostate cancer cell line du145  (Cancer Research Technology Limited)

Journal: Oncology Research

Article Title: Elucidating the Potential Targets and Mechanisms of Bisphenol A-Induced Prostate Cancer Based on Network Toxicology and Molecular Docking Analyses

doi: 10.32604/or.2026.076716

Figure Lengend Snippet: BPA promotes the migration and invasion of PC3 and DU145 cells. ( A ) PC3 and DU145 cells were seeded in the upper chamber of Transwell devices and treated with BPA for 24 h, followed by crystal violet staining of migrated cells. ( B ) Cells were seeded in the upper chamber of Transwell devices coated with Matrigel and allowed to invade for 24 h, after which, the cells were stained and counted. Results are expressed as the means ± SD of three replicate experiments. ** p < 0.01, *** p < 0.001.

Article Snippet: PC3 and DU145 human prostate cancer cell lines were obtained from Shanghai Model Organisms Company (Shanghai, China; catalog no. NM-H003 and NM-H004).

Techniques: Migration, Staining

Journal: Oncology Research

Article Title: Elucidating the Potential Targets and Mechanisms of Bisphenol A-Induced Prostate Cancer Based on Network Toxicology and Molecular Docking Analyses

doi: 10.32604/or.2026.076716

Figure Lengend Snippet: BPA regulates protein levels of EMT biomarkers and transcription factors in prostate cancer cells. ( A , B ) After 24 h of BPA treatment, the expression levels of N-cadherin, vimentin, MMP2, MMP9, and MT1-MMP were measured in PC3 and DU145 cells. ( C , D ) After 24 h of BPA treatment, the expression levels of Snail, Slug, and Twist1 were measured in PC3 and DU145 cells. Data are shown as the mean ± standard deviation of three independent experiments. ** p < 0.01, *** p < 0.001.

Article Snippet: PC3 and DU145 human prostate cancer cell lines were obtained from Shanghai Model Organisms Company (Shanghai, China; catalog no. NM-H003 and NM-H004).

Techniques: Expressing, Standard Deviation

Journal: Oncology Research

Article Title: Elucidating the Potential Targets and Mechanisms of Bisphenol A-Induced Prostate Cancer Based on Network Toxicology and Molecular Docking Analyses

doi: 10.32604/or.2026.076716

Figure Lengend Snippet: BPA activates the PI3K/AKT/GSK-3β/β-catenin signaling pathway.( A , B ) BPA enhanced the PI3K/AKT/GSK-3β/β-catenin signaling pathway in a dose-dependent manner; cells were treated with BPA at different concentrations for 24 h. ( C , D ) BPA reversed the effect of the PI3K inhibitor LY294002 on the PI3K/AKT/GSK-3β/β-catenin signaling pathway in prostate cancer cells. Data are shown as the mean ± standard deviation of three independent experiments. ** p < 0.01, *** p < 0.001.

Article Snippet: PC3 and DU145 human prostate cancer cell lines were obtained from Shanghai Model Organisms Company (Shanghai, China; catalog no. NM-H003 and NM-H004).

Techniques: Standard Deviation

Journal: Oncology Research

Article Title: Elucidating the Potential Targets and Mechanisms of Bisphenol A-Induced Prostate Cancer Based on Network Toxicology and Molecular Docking Analyses

doi: 10.32604/or.2026.076716

Figure Lengend Snippet: BPA promotes the migration and invasion of PC3 and DU145 cells. ( A ) PC3 and DU145 cells were seeded in the upper chamber of Transwell devices and treated with BPA for 24 h, followed by crystal violet staining of migrated cells. ( B ) Cells were seeded in the upper chamber of Transwell devices coated with Matrigel and allowed to invade for 24 h, after which, the cells were stained and counted. Results are expressed as the means ± SD of three replicate experiments. *** p < 0.001.

Article Snippet: PC3 and DU145 human prostate cancer cell lines were obtained from Shanghai Model Organisms Company (Shanghai, China; catalog no. NM-H003 and NM-H004).

Techniques: Migration, Staining